Peptide NGlycosidase F, also known as PNGase F, is an amidase specifically remove asparagine-linked (N-linked) polysaccharides from glycoproteins.
1. proteomic analysis: MALDITOF mass spectrometry; identification of N-linked glycosylation sites.
2. facilitate crystallization of glycoproteins.
Biological Source: Recombinant PNGase F is a glycosidase cloned from Elizabethkingia miricola (formerly Flavobacterium meningosepticum ) and expressed in E. coli .
Concentration: 10,000 units/ml. Molecular Weight: PNGase F has a molecular weight of approximately 36 kDa. Physical Form: PNGase F is supplied in 20 mM TrisHCl (pH 7.5 at 25°C), 50 mM NaCl, and 5mM EDTA. Storage: Stored at 4-8 °C.
Unit Definition: One unit of PNGase F will catalyze the deglycosylation of 1 nanomole of denatured Ribonuclease B (RNase B) in one minute at 37°C. One unit is equal to 1 IUB milliunit.