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PNGase F Biomiga Headquarters

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PNGase F
Features :
Price : $79.00
Quantity :
Catalogue#: PN1101-00
  
 

Description :

Description :

Peptide NGlycosidase F, also known as PNGase F, is an amidase specifically remove asparagine-linked (N-linked) polysaccharides from glycoproteins.

Applications :

1. proteomic analysis: MALDITOF mass spectrometry; identification of N-linked glycosylation sites.

2. facilitate crystallization of glycoproteins.

Specifications :

Properties:

Biological Source: Recombinant PNGase F is a glycosidase cloned from Elizabethkingia miricola (formerly Flavobacterium meningosepticum ) and expressed in E. coli .

Concentration: 10,000 units/ml. Molecular Weight: PNGase F has a molecular weight of approximately 36 kDa. Physical Form: PNGase F is supplied in 20 mM TrisHCl (pH 7.5 at 25°C), 50 mM NaCl, and 5mM EDTA. Storage: Stored at 4-8 °C.

Unit Definition: One unit of PNGase F will catalyze the deglycosylation of 1 nanomole of denatured Ribonuclease B (RNase B) in one minute at 37°C. One unit is equal to 1 IUB milliunit.