Hot Start Taq DNA polymerase is an antibody-inactivated hot-
start enzyme designed to block polymerase activity at ambient temperature. Once the PCR step reaches denaturation temperature (94℃),
Taq DNA polymerase activity is restored and the resulting PCR exhibits higher sensitivity, specificity and yield. The use of this antibody-
based hot start enzyme complex allows for convenient room temperature reaction setup and reduces PCR optimization effort and contamination risk.
Hot Start Taq DNA polymerase has intrinsic 5'->3' DNA polymerase activity and 5'->3' exonuclease activity but lacks 3'-
>5' exonuclease activity. It is a single polypeptide chain with a molecular weight of approximately 95 kDa and displays optimal activity at a
temperature between 70-74℃. The enzyme is provided with 10XPCR buffer and 50 mM MgCl2 solution to perform PCR amplification.