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Hot Start Taq DNA Polymerase

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Hot Start Taq DNA Polymerase
Features :
Price : $15.00
Quantity :
Catalogue#: HTP01-00
  
 

Description :

Description

 

Hot Start Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to block  polymerase  activity  at  ambient  temperature. 

Once  the  PCR  step  reaches denaturation  temperature (94℃), Taq  DNA  polymerase  activity  is  restored  and  the resulting PCR exhibits 

higher sensitivity, specificity and yield. The use of this antibody-based hot start enzyme complex allows for convenient room temperature reaction 

set-up and reduces PCR optimization effort and contamination risk.

Hot Start Taq DNA polymerase has intrinsic 5'->3' DNA polymerase activity and 5'->3' exonuclease activity but lacks 3'-

>5' exonuclease activity. It is a single polypeptide chain with  a  molecular  weight  of  approximately  95 kDa  and  displays  optimal  activity at 

a temperature between 70-74℃. The enzyme is provided with 10XPCR buffer and 50 mM MgCl2 solution to perform PCR amplification.