Biomiga provides researchers with a variety of ready-to-use cell line lysates for proteomics research. Biomiga lysates are available for applications including electrophoresis, western blot, immunoprecipitation, enzymatic activity analysis, protein-protein interaction analysis, and tissue specific expression identification. Please check product documentation to see recommended and tested applications for each cell line lysate.
HT1080 (Human fibrosarcoma cell line) Whole Cell Lysate
WB, ELISA, Immunoprecipitation
HT1080 cell lysate is ready to run on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.
HT1080 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylene diamine tetra acetic acid, 1 mM phenyl methyl sulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
Whole Cell Lysate
It is supplied as whole cell lysate, in RIPA buffer with protease inhibitors and phosphotase inhibitors.
Shipped on dry ice. Upon delivery aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Cell line: HT1080 (Human fibrosarcoma)
Growth media: DMEM & 10% FCS (Fetal Calf serum)
Figure 1. HT1080 cell morphology.
Figure 2. GAPDH western blot using HT1080 lysate.