Plasmid Midiprep: Isolate up to 500 µg plasmid DNA from 15 to 100 mL of culture.

Midiprep I: PD1411 and PD1413

• Purify up to 200 µg plasmid DNA from 15 to 50 mL culture.
• Choice of centrifugation or vacuum manifold methods.
• Choice of syringe filter or centrifugation to clear lysate.
• Centrifugation protocol: less than 60 minutes as compared to 2.5 hours of competitor.
• Manifold protocol: less than 45 minutes as compared to 2.5 hours competitor.

Midiprep II: PD1412 and PD1414

• Purify up to 500 µg plasmid DNA from 50 to 100 mL culture.
• Choice of centrifugation or vacuum manifold methods.
• Choice of syringe filter or centrifugation to clear lysate.
• Centrifugation protocol: less than 60 minutes as compared to 2.5 hours of competitor.
• Manifold protocol: less than 40 minutes as compared to 2.5 hours of competitor.