Plasmid Midiprep: Isolate up to 500 µg plasmid DNA from 15 to 100 mL of culture. Midiprep I: PD1411 and PD1413- Purify up to 200 µg plasmid DNA from 15 to 50 mL culture.
- Choice of centrifugation or vacuum manifold methods.
- Choice of syringe filter or centrifugation to clear lysate.
- Centrifugation protocol: less than 60 minutes as compared to 2.5 hours of competitor.
- Manifold protocol: less than 45 minutes as compared to 2.5 hours competitor.
Midiprep II: PD1412 and PD1414- Purify up to 500 µg plasmid DNA from 50 to 100 mL culture.
- Choice of centrifugation or vacuum manifold methods.
- Choice of syringe filter or centrifugation to clear lysate.
- Centrifugation protocol: less than 60 minutes as compared to 2.5 hours of competitor.
- Manifold protocol: less than 40 minutes as compared to 2.5 hours of competitor.
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